Peer-Reviewed Journal Details
Mandatory Fields
Rince, A,Tangney, M,Fitzgerald, GF;
2000
January
Fems Microbiology Letters
Identification of a DNA region from lactococcal phage sk1 protecting phage 712 from the abortive infection mechanism AbiF
Validated
()
Optional Fields
small isometric-headed phage 712 AbiF abortive infection mechanism PLASMID PNP40 LACTIS BACTERIOPHAGE-SK1 RESISTANCE TRANSCRIPTION STREPTOCOCCI SEQUENCE CLONING OPERON
182
185
191
Bacteriophage 712 is a small isometric-headed phage which is sensitive to the lactococcal abortive infection mechanism AbiF. Its 29.6-kb DNA genome was characterized by restriction mapping and transcriptional analysis. Construction of a gene bank of lactococcal phage ski, which is insensitive to the action of AbiF, in Lactococcus lactis containing AbiF resulted in the identification of a 324-bp DNA fragment which reduced the effect of the abortive infection mechanism on phage 712. Analysis of this region provided evidence that the action of AbiF is related to the cos ends of small isometric-headed phages. Sequence analysis of a 3.2-kb segment containing the middle operon and the cos ends of phage 712 genome allowed comparison of this part of the phage 712 genome with the equivalent sequences of four other small isometric-headed phages. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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