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Watson, D, Sleator, RD, Hill, C, Gahan, CGM;
BMC Microbiology
Enhancing Bile Tolerance Improves Survival and Persistence of Bifidobacterium and Lactococcus In The Murine Gastrointestinal Tract
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Results: In vitro bile tolerance of both strains was significantly enhanced (P < 0.001), following heterologous expression of the Listeria monocytogenes bile resistance mechanism BilE. Strains harbouring bilE were also recovered at significantly higher levels (P < 0.001), than control strains from the faeces and intestines of mice (n = 5), following oral inoculation. Furthermore, a B. breve strain expressing bilE demonstrated increased efficacy relative to the wild-type strain in reducing oral L. monocytogenes infection in mice.. Conclusion: Collectively the data indicates that bile tolerance can be enhanced in Bifidobacterium and Lactococcus species through rational. Background: The majority of commensal gastrointestinal bacteria used as probiotics are highly adapted to the specialised environment of the large bowel. However, unlike pathogenic bacteria; they are often inadequately equipped to endure the physicochemical stresses of gastrointestinal (GI) delivery in the host. Herein we outline a patho-biotechnology strategy to improve gastric delivery and host adaptation of a probiotic strain Bifidobacterium breve UCC2003 and the generally regarded as safe (GRAS) organism Lactococcus lactis NZ9000..
DOI 10.1186/1471-2180-8-176
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