Peer-Reviewed Journal Details
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Cronin, M, Knobel, M, O'Connell-Motherway, M, Fitzgerald, GF, van Sinderen, D;
2007
July
Applied and Environmental Microbiology
Molecular Dissection of A Bifidobacterial Replicon
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73
24
7858
7866
The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 106 transformants/mu g DNA..
DOI 10.1128/AEM.01630-07
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